Incubation:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
Immunohistochemical staining:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
Injection:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
Fluorescence:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
Expressing:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
Marker:Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Sections were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), anti-neurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 °C.
Article Title: Epidural Injection of Harpagoside for the Recovery of Rats with Lumbar Spinal Stenosis.
Article Snippet: Section were incubated with primary antibodies against rabbit anti-ectodermal dysplasia protein (ED1) (1:500; Millipore, Burlington, MA, USA), anti-iNOS (1:200; NovusBio, Littleton, CO, USA), anti-neuronal nuclear protein (NeuN) (1:500; Synaptic Systems, Göttingen, Germany), anti-transient receptor potential vanilloid type 1 channel (TRPV1) (1:100; Alomone Labs, Jerusalem, Israel), antineurofilament 200 (NF200) (1:200; Sigma-Aldrich, St. Louis, MO, USA), isolectin B4 (IB4) (1:100; Sigma-Aldrich), anti-calcitonin gene-related peptide (CGRP) (1:200; Sigma-Aldrich), and anti-5-hydroxytryptamine (5HT) (1:2000; Sigma-Aldrich) overnight at 4 ◦C.
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